Elicitation of immunity in mice after immunization with the S2 subunit of the severe acute respiratory syndrome coronavirus.
Identifieur interne : 004852 ( Main/Exploration ); précédent : 004851; suivant : 004853Elicitation of immunity in mice after immunization with the S2 subunit of the severe acute respiratory syndrome coronavirus.
Auteurs : Yingjun Guo [République populaire de Chine] ; Shuhan Sun ; Kaiyu Wang ; Shu Zhang ; Weijia Zhu ; Ze ChenSource :
- DNA and cell biology [ 1044-5498 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (sang), Cellules COS, Cellules Vero, Femelle, Fragments peptidiques (génétique), Fragments peptidiques (immunologie), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Humains, Immunisation, Immunoglobuline G (sang), Interféron gamma (biosynthèse), Interleukine-4 (biosynthèse), Lymphocytes T cytotoxiques (immunologie), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines recombinantes (immunologie), Souris, Souris de lignée BALB C, Virus du SRAS (immunologie).
- MESH :
- biosynthèse : Interféron gamma, Interleukine-4.
- génétique : Fragments peptidiques, Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- immunologie : Fragments peptidiques, Glycoprotéines membranaires, Lymphocytes T cytotoxiques, Protéines de l'enveloppe virale, Protéines recombinantes, Virus du SRAS.
- sang : Anticorps antiviraux, Immunoglobuline G.
- Animaux, Cellules COS, Cellules Vero, Femelle, Glycoprotéine de spicule des coronavirus, Humains, Immunisation, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (blood), COS Cells, Chlorocebus aethiops, Female, Humans, Immunization, Immunoglobulin G (blood), Interferon-gamma (biosynthesis), Interleukin-4 (biosynthesis), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Peptide Fragments (genetics), Peptide Fragments (immunology), Recombinant Proteins (immunology), SARS Virus (immunology), Spike Glycoprotein, Coronavirus, T-Lymphocytes, Cytotoxic (immunology), Vero Cells, Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , biosynthesis : Interferon-gamma, Interleukin-4.
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- chemical , genetics : Membrane Glycoproteins, Peptide Fragments, Viral Envelope Proteins.
- chemical , immunology : Membrane Glycoproteins, Peptide Fragments, Recombinant Proteins, Viral Envelope Proteins.
- immunology : SARS Virus, T-Lymphocytes, Cytotoxic.
- Animals, COS Cells, Chlorocebus aethiops, Female, Humans, Immunization, Mice, Mice, Inbred BALB C, Spike Glycoprotein, Coronavirus, Vero Cells.
Abstract
The S2 domain of the severe acute respiratory syndrome coronavirus (SARS-CoV) spike (S) protein is responsible for fusion between virus and target cell membranes, and is expected to be immungenic. In this study, we investigated the immune responses against the S2 subunit in BALB/c mice, which were vaccinated either with plasmid DNA encoding the S2 domain (residues 681-1120), the recombinant S2 fragment (residues 681-980) in incomplete Freund's adjuvant, or with inactivated SARS-CoV. The increased number of specific cytotoxic cells (CTLs) and the high titer of specific antibody showed stimulation of both arms of the immune system in these groups. The shift in cytokines suggested that Th1-polarized immune response was induced by plasmid pCoVS2, meanwhile the Th2-dominant response was induced by recombinant S2 fragment and inactivated vaccine. However, the titer of neutralizing antibodies was only detectable in mice immunized with inactivated virus, but not with pCoVS2 plasmid. Taken together, the S2 domain could induce specific cellular immune response and a high level of total IgG but little neutralizing antibodies against infection by SARSCoV.
DOI: 10.1089/dna.2005.24.510
PubMed: 16101349
Affiliations:
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Le document en format XML
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sortKey="Zhang, Shu" sort="Zhang, Shu" uniqKey="Zhang S" first="Shu" last="Zhang">Shu Zhang</name></author><author><name sortKey="Zhu, Weijia" sort="Zhu, Weijia" uniqKey="Zhu W" first="Weijia" last="Zhu">Weijia Zhu</name></author><author><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16101349</idno><idno type="pmid">16101349</idno><idno type="doi">10.1089/dna.2005.24.510</idno><idno type="wicri:Area/PubMed/Corpus">002592</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002592</idno><idno type="wicri:Area/PubMed/Curation">002592</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002592</idno><idno type="wicri:Area/PubMed/Checkpoint">002734</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" 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type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (blood)</term><term>COS Cells</term><term>Chlorocebus aethiops</term><term>Female</term><term>Humans</term><term>Immunization</term><term>Immunoglobulin G (blood)</term><term>Interferon-gamma (biosynthesis)</term><term>Interleukin-4 (biosynthesis)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Peptide Fragments (genetics)</term><term>Peptide Fragments (immunology)</term><term>Recombinant Proteins (immunology)</term><term>SARS Virus (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>T-Lymphocytes, Cytotoxic (immunology)</term><term>Vero Cells</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Cellules COS</term><term>Cellules Vero</term><term>Femelle</term><term>Fragments peptidiques (génétique)</term><term>Fragments peptidiques (immunologie)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Humains</term><term>Immunisation</term><term>Immunoglobuline G (sang)</term><term>Interféron gamma (biosynthèse)</term><term>Interleukine-4 (biosynthèse)</term><term>Lymphocytes T cytotoxiques (immunologie)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines recombinantes (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" 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xml:lang="fr"><term>Fragments peptidiques</term><term>Glycoprotéines membranaires</term><term>Lymphocytes T cytotoxiques</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>T-Lymphocytes, Cytotoxic</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>COS Cells</term><term>Chlorocebus aethiops</term><term>Female</term><term>Humans</term><term>Immunization</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Spike Glycoprotein, Coronavirus</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules COS</term><term>Cellules Vero</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Immunisation</term><term>Souris</term><term>Souris de lignée BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The S2 domain of the severe acute respiratory syndrome coronavirus (SARS-CoV) spike (S) protein is responsible for fusion between virus and target cell membranes, and is expected to be immungenic. In this study, we investigated the immune responses against the S2 subunit in BALB/c mice, which were vaccinated either with plasmid DNA encoding the S2 domain (residues 681-1120), the recombinant S2 fragment (residues 681-980) in incomplete Freund's adjuvant, or with inactivated SARS-CoV. The increased number of specific cytotoxic cells (CTLs) and the high titer of specific antibody showed stimulation of both arms of the immune system in these groups. The shift in cytokines suggested that Th1-polarized immune response was induced by plasmid pCoVS2, meanwhile the Th2-dominant response was induced by recombinant S2 fragment and inactivated vaccine. However, the titer of neutralizing antibodies was only detectable in mice immunized with inactivated virus, but not with pCoVS2 plasmid. Taken together, the S2 domain could induce specific cellular immune response and a high level of total IgG but little neutralizing antibodies against infection by SARSCoV.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><name sortKey="Sun, Shuhan" sort="Sun, Shuhan" uniqKey="Sun S" first="Shuhan" last="Sun">Shuhan Sun</name><name sortKey="Wang, Kaiyu" sort="Wang, Kaiyu" uniqKey="Wang K" first="Kaiyu" last="Wang">Kaiyu Wang</name><name sortKey="Zhang, Shu" sort="Zhang, Shu" uniqKey="Zhang S" first="Shu" last="Zhang">Shu Zhang</name><name sortKey="Zhu, Weijia" sort="Zhu, Weijia" uniqKey="Zhu W" first="Weijia" last="Zhu">Weijia Zhu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Guo, Yingjun" sort="Guo, Yingjun" uniqKey="Guo Y" first="Yingjun" last="Guo">Yingjun Guo</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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